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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been noticed in many tumors, which results in chromosome instability, and so may possibly lead to tumorigenesis. This protein was discovered to affiliate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a important part in promoting mobile-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two more splice variants had been claimed but detailed nucleotide sequence information will not be but available.
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Blocking peptides are peptides that bind exclusively to your focus on antibody and block antibody binding. These peptide typically has the epitope regarded through the antibody.
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This information outlines more feasible optimization of mobile and immunoassay parameters to ensure the best possible final results are...
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This tutorial outlines more attainable optimization of mobile and immunoassay parameters to guarantee the very best effects are...
Antibodies sure to the blocking peptide no more bind to your epitope over the target protein. By comparing the staining from the blocked antibody versus the antibody by itself, one can see which staining is specific.
Concentrate on Info Cyclin E belongs to your remarkably conserved cyclin relatives, whose associates are characterized by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins perform as regulators of CDK kinases. Diverse cyclins show distinct expression and degradation styles which lead on the temporal coordination of each mitotic function. Cyclin E forms a posh with and functions as a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. website This protein accumulates at the G1-S period boundary and is also degraded as cells development by S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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